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KMID : 0613820070170010132
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Journal of Life Science
2007 Volume.17 No. 1 p.132 ~ p.136
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Enhancement of Soluble Expression of Alginate Lyase By Molecular Chaperone in E. coli
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Shin Eun-Jung
Lee Jae-Hyung
Park So-Lim
Kim Hyeung-Rak
Nam Soo-Wan
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Abstract
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When alginate lyase gene (aly) from Pseudoalteromonas elyakovii was expressed in E. coli, most of the gene product was produced as aggregated insoluble particles known as inclusion bodies. In order to produce a soluble and active form of alginate lyase, E. coli cells were cotransformed with the plasmids designed to permit coexpression of aly together with molecular chaperones such as DnaK/DnaJ/GrpE or GroEL/ES chaperones. The results revealed that the coexpression of aly together with DnaK/DnaJ/GrpE chaperone had a marked effect on the production of this protein as a soluble and active form, presumably through facilitating correct folding of alginate lyase protein. The optimal concentration of L-arabinose for the induction of DnaK/DnaJ/GrpE chaperone was found to be 0.05 mg/ml. When DnaK/DnaJ/GrpE chaperone was coexpressed, about 34% in the total alginate lyase was produced in the soluble fraction. By addition of 10% cetylpyridinium chloride, a clear zone around the colony coexpressing aly and DnaK/DnaJ/GrpE chaperone was formed, indicating that the alginate in the medium was hydrolyzed by active alginate lyase enzyme.
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KEYWORD
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Alginate lyase, molecular chaperone, coexpression, E. coli
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